Amino acid count in known LC8 binding partner -8 -7 -6 -5 -4 -3 -2 -1 0 1 2 A 0 6 5 5 4 5 11 0 0 1 1 C 0 1 1 2 0 0 1 0 0 2 0 D 4 1 1 2 23 0 2 1 0 1 15 E 0 8 1 5 5 1 5 0 0 1 11 F 0 0 1 1 0 3 0 0 0 0 3 G 1 3 1 2 0 0 16 0 1 0 2 H 0 3 2 1 2 0 1 0 0 0 0 I 4 2 2 2 1 4 0 8 0 0 2 K 7 5 3 2 0 19 1 0 0 0 2 L 6 1 4 5 0 0 0 1 1 1 4 M 0 1 6 1 1 1 0 0 1 0 3 N 2 2 0 2 5 3 1 0 0 0 0 P 8 6 0 1 1 1 0 0 0 1 7 Q 2 4 3 5 0 4 6 0 61 0 2 R 4 5 2 5 0 9 1 0 1 0 0 S 9 9 11 4 13 5 17 7 1 0 5 T 9 3 15 4 11 5 4 43 0 55 3 V 10 4 6 11 0 4 0 6 0 4 5 W 0 1 0 1 0 0 0 0 0 0 0 Y 0 1 2 5 0 2 0 0 0 0 1 --------------------- Amino acid frequency in known LC8 binding partners with pseudo count -8 -7 -6 -5 -4 -3 -2 -1 0 1 2 A 0.658 8.553 7.237 7.237 5.921 7.237 15.132 0.658 0.658 1.974 1.974 C 0.658 1.974 1.974 3.289 0.658 0.658 1.974 0.658 0.658 3.289 0.658 D 5.921 1.974 1.974 3.289 30.921 0.658 3.289 1.974 0.658 1.974 20.395 E 0.658 11.184 1.974 7.237 7.237 1.974 7.237 0.658 0.658 1.974 15.132 F 0.658 0.658 1.974 1.974 0.658 4.605 0.658 0.658 0.658 0.658 4.605 G 1.974 4.605 1.974 3.289 0.658 0.658 21.711 0.658 1.974 0.658 3.289 H 0.658 4.605 3.289 1.974 3.289 0.658 1.974 0.658 0.658 0.658 0.658 I 5.921 3.289 3.289 3.289 1.974 5.921 0.658 11.184 0.658 0.658 3.289 K 9.868 7.237 4.605 3.289 0.658 25.658 1.974 0.658 0.658 0.658 3.289 L 8.553 1.974 5.921 7.237 0.658 0.658 0.658 1.974 1.974 1.974 5.921 M 0.658 1.974 8.553 1.974 1.974 1.974 0.658 0.658 1.974 0.658 4.605 N 3.289 3.289 0.658 3.289 7.237 4.605 1.974 0.658 0.658 0.658 0.658 P 11.184 8.553 0.658 1.974 1.974 1.974 0.658 0.658 0.658 1.974 9.868 Q 3.289 5.921 4.605 7.237 0.658 5.921 8.553 0.658 80.921 0.658 3.289 R 5.921 7.237 3.289 7.237 0.658 12.500 1.974 0.658 1.974 0.658 0.658 S 12.500 12.500 15.132 5.921 17.763 7.237 23.026 9.868 1.974 0.658 7.237 T 12.500 4.605 20.395 5.921 15.132 7.237 5.921 57.237 0.658 73.026 4.605 V 13.816 5.921 8.553 15.132 0.658 5.921 0.658 8.553 0.658 5.921 7.237 W 0.658 1.974 0.658 1.974 0.658 0.658 0.658 0.658 0.658 0.658 0.658 Y 0.658 1.974 3.289 7.237 0.658 3.289 0.658 0.658 0.658 0.658 1.974 --------------------- Random amino acid frequency in the eukariot proteome -8 -7 -6 -5 -4 -3 -2 -1 0 1 2 A 7.507 7.522 7.502 7.499 7.498 7.497 7.495 7.493 7.489 7.484 7.480 C 1.779 1.782 1.784 1.784 1.784 1.785 1.785 1.786 1.786 1.786 1.787 D 5.389 5.401 5.400 5.400 5.403 5.404 5.405 5.406 5.408 5.409 5.410 E 6.472 6.487 6.488 6.491 6.494 6.496 6.498 6.502 6.504 6.506 6.508 F 3.835 3.842 3.844 3.844 3.843 3.843 3.844 3.843 3.844 3.844 3.845 G 6.328 6.342 6.343 6.345 6.348 6.349 6.351 6.353 6.354 6.354 6.350 H 2.451 2.456 2.457 2.458 2.458 2.458 2.458 2.459 2.459 2.460 2.461 I 5.094 5.104 5.108 5.110 5.110 5.110 5.110 5.109 5.108 5.108 5.108 K 5.626 5.640 5.644 5.647 5.650 5.652 5.656 5.659 5.664 5.670 5.676 L 9.311 9.329 9.335 9.336 9.335 9.333 9.331 9.326 9.321 9.315 9.312 M 2.241 2.041 2.042 2.043 2.044 2.044 2.046 2.047 2.048 2.049 2.051 N 4.261 4.270 4.271 4.271 4.270 4.270 4.270 4.271 4.271 4.272 4.275 P 5.485 5.495 5.493 5.492 5.489 5.487 5.485 5.483 5.480 5.476 5.472 Q 4.253 4.262 4.265 4.266 4.265 4.265 4.266 4.266 4.266 4.267 4.268 R 5.662 5.675 5.680 5.680 5.680 5.679 5.680 5.680 5.680 5.682 5.683 S 8.365 8.381 8.368 8.359 8.354 8.350 8.346 8.344 8.343 8.342 8.340 T 5.620 5.630 5.628 5.624 5.621 5.618 5.616 5.614 5.613 5.612 5.609 V 6.182 6.194 6.197 6.199 6.200 6.201 6.200 6.200 6.199 6.196 6.196 W 1.220 1.223 1.224 1.225 1.226 1.226 1.227 1.228 1.228 1.229 1.230 Y 2.870 2.876 2.879 2.881 2.882 2.883 2.884 2.885 2.887 2.888 2.891 --------------------- PSSM -8 -7 -6 -5 -4 -3 -2 -1 0 1 2 A -1.057 0.056 -0.016 -0.015 -0.103 -0.015 0.305 -1.056 -1.056 -0.579 -0.579 C -0.432 0.044 0.044 0.266 -0.433 -0.433 0.044 -0.434 -0.434 0.265 -0.434 D 0.041 -0.437 -0.437 -0.215 0.758 -0.915 -0.216 -0.438 -0.915 -0.438 0.576 E -0.993 0.237 -0.517 0.047 0.047 -0.517 0.047 -0.995 -0.995 -0.518 0.366 F -0.766 -0.766 -0.290 -0.290 -0.767 0.079 -0.767 -0.767 -0.767 -0.767 0.078 G -0.506 -0.139 -0.507 -0.285 -0.984 -0.985 0.534 -0.985 -0.508 -0.985 -0.286 H -0.571 0.273 0.127 -0.095 0.127 -0.572 -0.095 -0.573 -0.573 -0.573 -0.573 I 0.065 -0.191 -0.191 -0.191 -0.413 0.064 -0.890 0.340 -0.890 -0.890 -0.191 K 0.244 0.108 -0.088 -0.235 -0.934 0.657 -0.457 -0.935 -0.935 -0.935 -0.237 L -0.037 -0.675 -0.198 -0.111 -1.152 -1.152 -1.152 -0.674 -0.674 -0.674 -0.197 M -0.532 -0.015 0.622 -0.015 -0.015 -0.015 -0.493 -0.493 -0.016 -0.493 0.351 N -0.112 -0.113 -0.812 -0.113 0.229 0.033 -0.335 -0.812 -0.812 -0.812 -0.813 P 0.309 0.192 -0.922 -0.444 -0.444 -0.444 -0.921 -0.921 -0.921 -0.443 0.256 Q -0.112 0.143 0.033 0.230 -0.812 0.142 0.302 -0.812 1.278 -0.812 -0.113 R 0.019 0.106 -0.237 0.105 -0.936 0.343 -0.459 -0.936 -0.459 -0.936 -0.936 S 0.174 0.174 0.257 -0.150 0.328 -0.062 0.441 0.073 -0.626 -1.103 -0.062 T 0.347 -0.087 0.559 0.022 0.430 0.110 0.023 1.008 -0.931 1.114 -0.086 V 0.349 -0.020 0.140 0.388 -0.974 -0.020 -0.974 0.140 -0.974 -0.020 0.067 W -0.268 0.208 -0.270 0.207 -0.270 -0.270 -0.271 -0.271 -0.271 -0.271 -0.272 Y -0.640 -0.164 0.058 0.400 -0.642 0.057 -0.642 -0.642 -0.642 -0.642 -0.166 ----------------- FAG PSSM (Amino acid frequency in the phage display in %) -7 -6 -5 -4 -3 -2 -1 0 1 2 A 3 6 0 2 8 18 0 0 1 1 C 0 0 0 0 0 0 0 0 0 0 D 0 0 0 26 0 0 0 0 0 9 E 0 0 0 0 0 0 0 0 0 20 F 6 18 0 0 0 0 0 0 2 6 G 6 2 0 0 0 25 1 0 0 3 H 0 3 9 4 0 6 0 0 0 6 I 3 0 15 0 23 0 12 0 0 6 K 9 3 15 0 23 0 0 0 0 0 L 4 10 2 0 0 0 1 0 0 3 M 15 33 9 0 3 0 2 11 0 0 N 15 0 15 11 0 0 0 2 0 3 P 2 0 0 2 0 0 0 0 0 1 Q 3 0 0 0 0 3 0 87 0 0 R 9 7 15 0 12 0 0 0 0 3 S 6 5 0 27 2 6 2 0 3 8 T 0 3 2 27 0 0 71 0 85 7 V 14 5 18 2 29 2 11 0 9 6 Y 3 6 0 0 0 3 0 0 0 14 W 0 0 0 0 0 37 0 0 0 6